Lab in Molecular Biology

Enrollment limited to 12. Lab fee: $150. Prerequisites: one year of biology (BIOL UN2005 and BIOL UN2006) and Contemporary Biology Laboratory (BIOL UN2501). Prerequisites: one year of biology (UN2005-UN2006) and Contemporary Biology Laboratory (UN2501). This lab will explore various molecular biology techniques frequently utilized in modern molecular biology laboratories. The lab will consist of four modules: 1) Molecular verification of genetically modified organisms (GMOs); 2) Site-directed mutagenesis; 3) gDNA extraction, PCR amplification, sequencing and GenBank analysis of the COI genes from diverse fish species and 4) protein gel analysis of fish muscle components. SPS and TC students may register for this course, but they must first obtain the written permission of the instructor, by filling out a paper Registration Adjustment Form (Add/Drop form). The form can be downloaded at: http://registrar.columbia.edu/sites/default/files/content/reg-adjustment.pdf